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Field	Query

	Field	Term

General Information:

Id:	685
Diseases:	Parkinson disease
Organism:	Mus musculus
Tissue/Cell line:	SN4741 cell; BTO:0003945 MN9D cell
Publication type:	article
Reference:	Heo HY et al.(2010) LRRK2 enhances oxidative stress-induced neurotoxicity via its kinase activity Exp. Cell Res. 316: 649-656 [PMID: 19769964]

Interaction Information:

Comment	Overexpression of LRRK2-WT, PD-linked mutant LRRK2-G2019S in SN4741 cells cause cell death which was modestly (WT) or significantly (G2019S) increased. When these transfected cells were treated with hydrogen peroxide to mimic oxidative stress, cellular neurotoxicity was enhanced in the same order.
Formal Description Interaction-ID: 3706	gene/protein LRRK2 increases_activity of process neuron death if overexpressed

Comment	Overexpression of LRRK2-WT, PD-linked mutant LRRK2-G2019S in SN4741 cells cause cell death which was modestly (WT) or significantly (G2019S) increased. When these transfected cells were treated with hydrogen peroxide to mimic oxidative stress, cellular neurotoxicity was enhanced in the same order.
Formal Description Interaction-ID: 3714	gene/protein mutant LRRK2-p.G2019S increases_activity of process neuron death cell death was increased with a higher rate than wild type

Comment	Overexpression of LRRK2-WT, PD-linked mutant LRRK2-G2019S in SN4741 cells cause cell death which was modestly (WT) or significantly (G2019S) increased. When these transfected cells were treated with hydrogen peroxide to mimic oxidative stress, cellular neurotoxicity was enhanced in the same order.
Formal Description Interaction-ID: 3717	drug/chemical compound H2O2 increases_activity of process neuron death

Comment	Overexpression of LRRK2-WT, PD-linked mutant LRRK2-G2019S in SN4741 cells cause cell death which was modestly (WT) or significantly (G2019S) increased. When these transfected cells were treated with hydrogen peroxide to mimic oxidative stress, cellular neurotoxicity was enhanced in the same order.
Formal Description Interaction-ID: 3719	gene/protein LRRK2 increases_activity of process response to oxidative stress if overexpressed

Comment	Overexpression of LRRK2-WT, PD-linked mutant LRRK2-G2019S in SN4741 cells cause cell death which was modestly (WT) or significantly (G2019S) increased. When these transfected cells were treated with hydrogen peroxide to mimic oxidative stress, cellular neurotoxicity was enhanced in the same order.
Formal Description Interaction-ID: 3720	gene/protein mutant LRRK2-p.G2019S increases_activity of process response to oxidative stress cell death was increased with a higher rate than wild type

Comment	Over-expression of LRRK2-WT and PD-linked mutant LRRK2-G2019S lead to a weak, but significant, increase in intracellular reactive oxygen species (ROS). G2019S enhances this effect.
Formal Description Interaction-ID: 3723	gene/protein LRRK2 increases_quantity of drug/chemical compound Reactive oxygen species if overexpressed

Comment	Over-expression of LRRK2-WT and PD-linked mutant LRRK2-G2019S lead to a weak, but significant, increase in intracellular reactive oxygen species (ROS). G2019S enhances this effect.
Formal Description Interaction-ID: 3725	gene/protein mutant LRRK2-p.G2019S increases_quantity of drug/chemical compound Reactive oxygen species amount of ROS was increased even with a higher rate than wild type

Comment	Transfection of DJ-1 WT increased survival rate under hydrogen peroxide treatment regardless of whether the vector, LRRK2 WT or LRRK2-G2019S was co-transfected. However, the increase in survival rate was most remarkable in cells expressing G2019S under oxidative stress, which almost eradicated G2019S neurotoxicity and showed a cell survival rate similar to that of cells transfected with the control vectors.
Formal Description Interaction-ID: 3731	gene/protein PARK7 decreases_activity of process neuron death induced by hydrogen peroxide treatment
Drugbank entries	Show/Hide entries for PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7

Comment	Transfection of DJ-1 WT increased survival rate under hydrogen peroxide treatment regardless of whether the vector, LRRK2 WT or LRRK2-G2019S was co-transfected. However, the increase in survival rate was most remarkable in cells expressing G2019S under oxidative stress, which almost eradicated G2019Ss neurotoxicity and showed a cell survival rate similar to that of cells transfected with the control vectors.
Formal Description Interaction-ID: 3733	gene/protein PARK7 decreases_activity of process neuron death induced by LRRK2-G2019S expression
Drugbank entries	Show/Hide entries for PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7

Comment	Transfection of DJ-1 WT increased survival rate under hydrogen peroxide treatment regardless of whether the vector, LRRK2 WT or LRRK2-G2019S was co-transfected. However, the increase in survival rate was most remarkable in cells expressing G2019S under oxidative stress, which almost eradicated G2019Ss neurotoxicity and showed a cell survival rate similar to that of cells transfected with the control vectors.
Formal Description Interaction-ID: 3734	gene/protein PARK7 decreases_activity of process response to oxidative stress
Drugbank entries	Show/Hide entries for PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7

Comment	PD98059 is a specific inhibitor of MEK1, a kinase involved in the ERK1 and ERK2 cascade.
Formal Description Interaction-ID: 3735	drug/chemical compound PD98059 decreases_activity of gene/protein MAP2K1
Drugbank entries	Show/Hide entries for MAP2K1
Drugbank DB02152	K-252a not specified MAP2K1
Drugbank DB03115	5-Bromo-N-(2,3-Dihydroxypropoxy)-3,4-Dif ... not specified MAP2K1
Drugbank DB06892	(5S)-4,5-difluoro-6-[(2-fluoro-4-iodophe ... not specified MAP2K1
Drugbank DB07046	2-[(2-chloro-4-iodophenyl)amino]-N-{[(2R ... not specified MAP2K1
Drugbank DB07101	N-{[(2R)-2,3-dihydroxypropyl]oxy}-3,4-di ... not specified MAP2K1
Drugbank DB08130	N-(5-{3,4-difluoro-2-[(2-fluoro-4-iodoph ... not specified MAP2K1
Drugbank DB08208	2-[(4-ETHYNYL-2-FLUOROPHENYL)AMINO]-3,4- ... not specified MAP2K1
Drugbank DB03115	5-Bromo-N-(2,3-Dihydroxypropoxy)-3,4-Dif ... not specified MAP2K1

Comment	PD98059 is a specific inhibitor of MEK1, a kinase involved in the ERK1 and ERK2 cascade.
Formal Description Interaction-ID: 3736	drug/chemical compound PD98059 decreases_activity of process ERK1 and ERK2 cascade via inhibiting MEK1

Comment	Treatment of PD98059, a specific inhibitor of MEK1, 1 h before hydrogen peroxide addition almost eliminated LRRK2-p.G2019S neurotoxicity.
Formal Description Interaction-ID: 3737	drug/chemical compound PD98059 decreases_activity of process neuron death induced by LRRK2-G2019S expression

Comment	Treatment of PD98059, a specific inhibitor of MEK1, 1 h before hydrogen peroxide addition almost eliminated LRRK2-p.G2019S neurotoxicity.
Formal Description Interaction-ID: 3738	drug/chemical compound PD98059 decreases_activity of gene/protein mutant LRRK2-p.G2019S

Comment	Transfection of DJ-1 WT increased survival rate under hydrogen peroxide treatment regardless of whether the vector, LRRK2 WT or LRRK2-G2019S was co-transfected. However, the increase in survival rate was most remarkable in cells expressing G2019S under oxidative stress, which almost eradicated LRRK2-G2019S neurotoxicity and showed a cell survival rate similar to that of cells transfected with the control vectors.
Formal Description Interaction-ID: 3739	gene/protein PARK7 decreases_activity of gene/protein mutant LRRK2-p.G2019S
Drugbank entries	Show/Hide entries for PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7

Comment	Transfection of DJ-1 WT increased survival rate under hydrogen peroxide treatment regardless of whether the vector, LRRK2 WT or LRRK2-G2019S was co-transfected. However, the increase in survival rate was most remarkable in cells expressing G2019S under oxidative stress, which almost eradicated LRRK2-G2019S neurotoxicity and showed a cell survival rate similar to that of cells transfected with the control vectors.
Formal Description Interaction-ID: 3740	gene/protein PARK7 decreases_quantity of drug/chemical compound H2O2
Drugbank entries	Show/Hide entries for PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7
Drugbank DB01769	Double Oxidized Cysteine not specified PARK7

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