CIDeRplusGeneral Information:
| Id: | 655 |
| Diseases: |
Amyotrophic lateral sclerosis
|
| Mus musculus | |
| BTO:0004488 NSC-34 cell | |
| article | |
| Reference: | Kupershmidt L et al.(2009) Neuroprotective and neuritogenic activities of novel multimodal iron-chelating drugs in motor-neuron-like NSC-34 cells and transgenic mouse model of amyotrophic lateral sclerosis FASEB J. 23: 3766-3779 [PMID: 19638399] |
Interaction Information:
| Comment | Treatment of NSC-34 cells with the iron-chelating drugs, M30 and HLA20 significantly reduced cell mortality induced by H2O2 and SIN-1 (3-Morpholinosydnonimine, a potent peroxynitrite ion generator). In these concentrations, M30 and HLA20 alone, in the absence of the neurotoxins, had no effect on cell viability, relative to control. |
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Formal Description Interaction-ID: 3471 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) decreases_activity of process |
| Comment | Treatment of NSC-34 cells with the iron-chelating drugs, M30 and HLA20 significantly reduced cell mortality induced by H2O2 and SIN-1 (3-Morpholinosydnonimine, a potent peroxynitrite ion generator). In these concentrations, M30 and HLA20 alone, in the absence of the neurotoxins, had no effect on cell viability, relative to control. |
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Formal Description Interaction-ID: 3486 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) decreases_activity of process |
| Comment | M30 and HLA20 induced a dose-dependent increase in TfR levels in, confirming the iron chelation effect of these drugs in NSC-34 cells. |
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Formal Description Interaction-ID: 3487 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_quantity of gene/protein |
| Comment | M30 and HLA20 induced a dose-dependent increase in TfR levels in, confirming the iron chelation effect of these drugs in NSC-34 cells. |
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Formal Description Interaction-ID: 3488 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_quantity of gene/protein |
| Comment | The levels of both mRNA and protein expression of HIF-1 alpha were increased in M30- and HLA20-treated NSC-34 cells, indicating that the drugs not only stabilized HIF-1 alpha protein but influenced its regulation on the transcriptional level. |
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Formal Description Interaction-ID: 3489 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Drugbank entries | Show/Hide entries for HIF1A |
| Comment | The levels of both mRNA and protein expression of HIF-1 alpha were increased in M30- and HLA20-treated NSC-34 cells, indicating that the drugs not only stabilized HIF-1 alpha protein but influenced its regulation on the transcriptional level. |
|
Formal Description Interaction-ID: 3490 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_quantity of gene/protein |
| Drugbank entries | Show/Hide entries for HIF1A |
| Comment | The levels of both mRNA and protein expression of HIF-1 alpha were increased in M30- and HLA20-treated NSC-34 cells, indicating that the drugs not only stabilized HIF-1 alpha protein but influenced its regulation on the transcriptional level. |
|
Formal Description Interaction-ID: 3491 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Drugbank entries | Show/Hide entries for HIF1A |
| Comment | The levels of both mRNA and protein expression of HIF-1 alpha were increased in M30- and HLA20-treated NSC-34 cells, indicating that the drugs not only stabilized HIF-1 alpha protein but influenced its regulation on the transcriptional level. |
|
Formal Description Interaction-ID: 3492 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_quantity of gene/protein |
| Drugbank entries | Show/Hide entries for HIF1A |
| Comment | Exposure of NSC-34 cells to M30 or HLA20 significantly increased mRNA levels of VEGF and enolase 1, two known HIF-1-regulated genes. |
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Formal Description Interaction-ID: 3493 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | Exposure of NSC-34 cells to M30 or HLA20 significantly increased mRNA levels of VEGF and enolase 1, two known HIF-1-regulated genes. |
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Formal Description Interaction-ID: 3494 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | Exposure of NSC-34 cells to M30 or HLA20 significantly increased mRNA levels of VEGF and enolase 1, two known HIF-1-regulated genes. |
|
Formal Description Interaction-ID: 3495 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | Exposure of NSC-34 cells to M30 or HLA20 significantly increased mRNA levels of VEGF and enolase 1, two known HIF-1-regulated genes. |
|
Formal Description Interaction-ID: 3496 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | M30 and HLA20 markedly increased the amount of phospho-AKT at 30 min of drug exposure. |
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Formal Description Interaction-ID: 3497 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_activity of gene/protein |
| Drugbank entries | Show/Hide entries for AKT1 |
| Comment | M30 and HLA20 markedly increased the amount of phospho-AKT at 30 min of drug exposure. |
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Formal Description Interaction-ID: 3498 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for AKT1 |
| Comment | M30 and HLA20 markedly increased the amount of phospho-AKT at 30 min of drug exposure. |
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Formal Description Interaction-ID: 3499 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_activity of gene/protein |
| Drugbank entries | Show/Hide entries for AKT1 |
| Comment | M30 and HLA20 markedly increased the amount of phospho-AKT at 30 min of drug exposure. |
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Formal Description Interaction-ID: 3500 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for AKT1 |
| Comment | M30 and HLA20 significantly increased phosphorylation of GSK-3-beta at Ser-9 in NSC-34 cells, suggesting that both drugs induced activation of AKT followed by phosphorylation (inactivation) of GSK-3-beta. |
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Formal Description Interaction-ID: 3501 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for GSK3B |
| Comment | M30 and HLA20 significantly increased phosphorylation of GSK-3-beta at Ser-9 in NSC-34 cells, suggesting that both drugs induced activation of AKT followed by phosphorylation (inactivation) of GSK-3-beta. |
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Formal Description Interaction-ID: 3529 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) decreases_activity of gene/protein |
| Drugbank entries | Show/Hide entries for GSK3B |
| Comment | M30 and HLA20 significantly increased phosphorylation of GSK-3-beta at Ser-9 in NSC-34 cells, suggesting that both drugs induced activation of AKT followed by phosphorylation (inactivation) of GSK-3-beta. |
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Formal Description Interaction-ID: 3530 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for GSK3B |
| Comment | M30 and HLA20 significantly increased phosphorylation of GSK-3-beta at Ser-9 in NSC-34 cells, suggesting that both drugs induced activation of AKT followed by phosphorylation (inactivation) of GSK-3-beta. |
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Formal Description Interaction-ID: 3531 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) decreases_activity of gene/protein |
| Drugbank entries | Show/Hide entries for GSK3B |
| Comment | M30 and HLA20 induced NSC-34 neuritogenesis, accompanied by a marked increase in the expression of brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43). |
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Formal Description Interaction-ID: 3534 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | M30 and HLA20 induced NSC-34 neuritogenesis, accompanied by a marked increase in the expression of brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43). |
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Formal Description Interaction-ID: 3535 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | M30 and HLA20 induced NSC-34 neuritogenesis, accompanied by a marked increase in the expression of brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43). |
|
Formal Description Interaction-ID: 3536 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | M30 and HLA20 induced NSC-34 neuritogenesis, accompanied by a marked increase in the expression of brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43). |
|
Formal Description Interaction-ID: 3537 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_expression of gene/protein |
| Comment | M30 and HLA20 markedly reduced the levels of cyclin D1. |
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Formal Description Interaction-ID: 3538 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) decreases_quantity of gene/protein |
| Drugbank entries | Show/Hide entries for CCND1 |
| Comment | M30 and HLA20 markedly reduced the levels of cyclin D1. |
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Formal Description Interaction-ID: 3539 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) decreases_quantity of gene/protein |
| Drugbank entries | Show/Hide entries for CCND1 |
| Comment | M30 and HLA20 caused a rapid phosphorylation of PKC and ERK1/2 in NSC-34 cells at 10 min, reaching a maximum at 20 min. |
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Formal Description Interaction-ID: 3540 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for MAPK3 |
| Comment | M30 and HLA20 caused a rapid phosphorylation of PKC and ERK1/2 in NSC-34 cells at 10 min, reaching a maximum at 20 min. |
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Formal Description Interaction-ID: 3541 |
drug/chemical compound (5-[N-methyl-N-propargylaminomethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for MAPK1 |
| Comment | M30 and HLA20 caused a rapid phosphorylation of PKC and ERK1/2 in NSC-34 cells at 10 min, reaching a maximum at 20 min. |
|
Formal Description Interaction-ID: 3542 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for MAPK3 |
| Comment | M30 and HLA20 caused a rapid phosphorylation of PKC and ERK1/2 in NSC-34 cells at 10 min, reaching a maximum at 20 min. |
|
Formal Description Interaction-ID: 3543 |
drug/chemical compound (5-[4-propargylpiperazin-1-ylmethyl]-8-hydroxyquinoline) increases_phosphorylation of gene/protein |
| Drugbank entries | Show/Hide entries for MAPK1 |