General Information:

Id: 627
Diseases: Amyotrophic lateral sclerosis
Rattus norvegicus
BTO:0001279 spinal cord
article
Reference: Tolosa L et al.(2008) Vascular endothelial growth factor protects spinal cord motoneurons against glutamate-induced excitotoxicity via phosphatidylinositol 3-kinase. J. Neurochem. 105: 1080-1090 [PMID: 18182045]

Interaction Information:

Comment Western blot analysis showed the presence of VEGFR2, but not VEGFR1 in the spinal cord organotypic cultures. VEGFR2 is specifically located in large neurons in the ventral horn of the spinal cord that were identified as motoneurons.
Formal Description
Interaction-ID: 3322

gene/protein

KDR

is_expressed_in

tissue/cell line

motoneuron

in spinal cord
Drugbank entries Show/Hide entries for KDR
Comment Western blot analysis showed the presence of VEGFR2, but not VEGFR1 in the spinal cord organotypic cultures. VEGFR2 is specifically located in large neurons in the ventral horn of the spinal cord that were identified as motoneurons.
Formal Description
Interaction-ID: 3323

gene/protein

FLT1

NOT is_expressed_in

tissue/cell line

motoneuron

in spinal cord
Drugbank entries Show/Hide entries for FLT1
Comment VEGF protects motoneurons against chronic glutamate excitotoxicity. VEGF significantly protected spinal cord motoneurons from excitotoxic death.
Formal Description
Interaction-ID: 3324

gene/protein

VEGFA

increases_activity of

phenotype

decreased susceptibility to neuronal excitotoxicity

in spinal cord motoneurons; exposed to THA (a glutamate transporter inhibitor) to induce chronic glutamate neurotoxicity
Drugbank entries Show/Hide entries for VEGFA
Comment VEGF protects motoneurons against chronic glutamate excitotoxicity. VEGF significantly protected spinal cord motoneurons from excitotoxic death.
Formal Description
Interaction-ID: 3325

gene/protein

VEGFA

decreases_activity of

phenotype

motor neuron degeneration

in spinal cord motoneurons; exposed to THA (a glutamate transporter inhibitor) to induce chronic glutamate neurotoxicity
Drugbank entries Show/Hide entries for VEGFA
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3326

gene/protein

VEGFA

increases_activity of

gene/protein

MAPK3

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or MAPK3
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3327

gene/protein

VEGFA

increases_phosphorylation of

gene/protein

MAPK3

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or MAPK3
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3328

gene/protein

VEGFA

increases_activity of

gene/protein

MAPK1

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or MAPK1
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3329

gene/protein

VEGFA

increases_phosphorylation of

gene/protein

MAPK1

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or MAPK1
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3330

gene/protein

VEGFA

increases_activity of

gene/protein

AKT1

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or AKT1
Comment VEGF stimulation induces an increase in the phosphorylation of both Akt and ERK, with a maximal activation of both kinases after 12 h of exposure.
Formal Description
Interaction-ID: 3331

gene/protein

VEGFA

increases_phosphorylation of

gene/protein

AKT1

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA or AKT1
Comment The phosphorylation of Akt and ERK stimulated by VEGF was abrogated in the presence of the PI3-K inhibitor, LY294002 or the MEK inhibitor, U0126, respectively; indicating that VEGF activates these kinases via PI3-K and MEK, respectively.
Formal Description
Interaction-ID: 3332

gene/protein

VEGFA

increases_activity of

process

phosphatidylinositol 3-kinase signaling

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA
Comment The phosphorylation of Akt and ERK stimulated by VEGF was abrogated in the presence of the PI3-K inhibitor, LY294002 or the MEK inhibitor, U0126, respectively; indicating that VEGF activates these kinases via PI3-K and MEK, respectively.
Formal Description
Interaction-ID: 3333

gene/protein

VEGFA

increases_activity of

process

MAPK cascade

in spinal cord motoneurons
Drugbank entries Show/Hide entries for VEGFA
Comment VEGF mediates neuroprotection against chronic glutamate-induced neurotoxicity and restores the levels of Bcl-2 and survivin via PI3-K/Akt. VEGF restores Bcl-2 levels after chronic glutamate excitotoxicty.
Formal Description
Interaction-ID: 3334

gene/protein

VEGFA

increases_quantity of

gene/protein

BCL2

in spinal cord motoneurons; exposed to THA (a glutamate transporter inhibitor) to induce chronic glutamate neurotoxicity
Drugbank entries Show/Hide entries for VEGFA or BCL2
Comment VEGF mediates neuroprotection against chronic glutamate-induced neurotoxicity and restores the levels of Bcl-2 and survivin via PI3-K/Akt. VEGF restores survivin levels after chronic glutamate excitotoxicty.
Formal Description
Interaction-ID: 3335

gene/protein

VEGFA

increases_quantity of

gene/protein

BIRC5

in spinal cord neurons; exposed to THA (a glutamate transporter inhibitor) to induce chronic glutamate neurotoxicity
Drugbank entries Show/Hide entries for VEGFA