CIDeRplusGeneral Information:
| Id: | 622 |
| Diseases: |
Amyotrophic lateral sclerosis
|
| Rattus norvegicus | |
| BTO:0001279 spinal cord | |
| article | |
| Reference: | Tolosa L et al.(2009) Vascular endothelial growth factor protects motoneurons from serum deprivation-induced cell death through phosphatidylinositol 3-kinase-mediated p38 mitogen-activated protein kinase inhibition. Neuroscience 158: 1348-1355 [PMID: 19041930] |
Interaction Information:
| Comment | VEGF prevented apoptotic cell death induced by serum withdrawal in dissociated spinal cord cultures. VEGF attenuated the cell loss and the increase in apoptotic nuclei, partially prevented the loss of neurofilament heavy chain protein and the fragmentation of PARP (a substrate of caspase-3 used as an indicator of apoptosis). |
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Formal Description Interaction-ID: 3286 |
gene/protein decreases_activity of process |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | Serum deprivation caused a significant loss (35%) of motoneurons that was abrogated in the presence of VEGF. |
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Formal Description Interaction-ID: 3287 |
process increases_activity of process |
| Comment | Serum deprivation caused a significant loss (35%) of motoneurons that was abrogated in the presence of VEGF. |
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Formal Description Interaction-ID: 3289 |
process increases_activity of phenotype |
| Comment | Serum deprivation caused a significant loss (35%) of motoneurons that was abrogated in the presence of VEGF. |
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Formal Description Interaction-ID: 3290 |
gene/protein decreases_activity of phenotype |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | Akt phosphorylation was enhanced in the presence of VEGF in serum-deprived cultures. |
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Formal Description Interaction-ID: 3293 |
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| Drugbank entries | Show/Hide entries for VEGFA or AKT1 |
| Comment | Akt phosphorylation was enhanced in the presence of VEGF in serum-deprived cultures. |
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Formal Description Interaction-ID: 3300 |
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| Drugbank entries | Show/Hide entries for VEGFA or AKT1 |
| Comment | Serum deprivation led to an increase in p38MAPK phosphorylation as compared with non-deprived cultures, which was blocked by the p38MAPK inhibitor SB203580. |
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Formal Description Interaction-ID: 3302 |
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| Comment | Serum deprivation led to an increase in p38MAPK phosphorylation as compared with non-deprived cultures, which was blocked by the p38MAPK inhibitor SB203580. |
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Formal Description Interaction-ID: 3305 |
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| Comment | The activation of p38MAPK after serum deprivation in spinal cord organotypic cultures was blocked in the presence of VEGF, thus indicating a prevention of the stress signal. The combination of the PI3-K inhibitor LY294002 plus VEGF restored the phosphorylation of p38MAPK induced by serum deprivation, indicating that VEGF acts through the PI3-K/Akt to inhibit the activation of p38MAPK in spinal cord cultures. |
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Formal Description Interaction-ID: 3306 |
gene/protein decreases_activity of gene/protein p38 MAPK |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | The activation of p38MAPK after serum deprivation in spinal cord organotypic cultures was blocked in the presence of VEGF, thus indicating a prevention of the stress signal. The combination of the PI3-K inhibitor LY294002 plus VEGF restored the phosphorylation of p38MAPK induced by serum deprivation, indicating that VEGF acts through the PI3-K/Akt to inhibit the activation of p38MAPK in spinal cord cultures. |
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Formal Description Interaction-ID: 3308 |
gene/protein decreases_phosphorylation of gene/protein p38 MAPK |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | The activation of p38MAPK after serum deprivation in spinal cord organotypic cultures was blocked in the presence of VEGF, thus indicating a prevention of the stress signal. The combination of the PI3-K inhibitor LY294002 plus VEGF restored the phosphorylation of p38MAPK induced by serum deprivation, indicating that VEGF acts through the PI3-K/Akt to inhibit the activation of p38MAPK in spinal cord cultures. |
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Formal Description Interaction-ID: 3312 |
gene/protein affects_activity of |
| Drugbank entries | Show/Hide entries for VEGFA |
| Comment | Bcl-2 expression was decreased after serum deprivation, and VEGF exposure restored the levels of this anti-apoptotic protein. |
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Formal Description Interaction-ID: 3313 |
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| Drugbank entries | Show/Hide entries for BCL2 |
| Comment | Bcl-2 expression was decreased after serum deprivation, and VEGF exposure restored the levels of this anti-apoptotic protein. The results suggest that VEGF restores the levels of Bcl-2 in spinal cord cultures through the inhibition of p38MAPK via the PI3-K pathway. |
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Formal Description Interaction-ID: 3315 |
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| Drugbank entries | Show/Hide entries for VEGFA or BCL2 |