General Information:

Id: 1,182
Diseases: Amyotrophic lateral sclerosis
Rattus norvegicus
BTO:0000099 astrocyte
article
Reference: Cassina P et al.(2002) Peroxynitrite triggers a phenotypic transformation in spinal cord astrocytes that induces motor neuron apoptosis J. Neurosci. Res. 67: 21-29 [PMID: 11754077]

Interaction Information:

Comment Peroxynitrite exposure induces reactive morphological changes and iNOS expression in astrocyte cultures.
Formal Description
Interaction-ID: 7831

drug/chemical compound

Peroxynitrite

increases_activity of

phenotype

abnormal astrocyte morphology

in primary astrocyte monolayers
Comment Peroxynitrite exposure induces reactive morphological changes and iNOS expression in astrocyte cultures.
Formal Description
Interaction-ID: 7833

drug/chemical compound

Peroxynitrite

increases_expression of

gene/protein

NOS2

in primary astrocyte monolayers
Drugbank entries Show/Hide entries for NOS2
Comment Peroxynitrite exposure induces reactive morphological changes and iNOS expression in astrocyte cultures. Bacterial LPS (a well-known inflammatory stimulus) showed similar effects.
Formal Description
Interaction-ID: 7834

drug/chemical compound

Lipopolysaccharide

increases_activity of

phenotype

abnormal astrocyte morphology

in primary astrocyte monolayers
Comment Peroxynitrite exposure induces reactive morphological changes and iNOS expression in astrocyte cultures. Bacterial LPS (a well-known inflammatory stimulus) showed similar effects.
Formal Description
Interaction-ID: 7836

drug/chemical compound

Lipopolysaccharide

increases_expression of

gene/protein

NOS2

in primary astrocyte monolayers
Drugbank entries Show/Hide entries for NOS2
Comment The concentration of nitrite and nitrate accumulating in the culture media increased three- to fourfold following exposure to either peroxynitrite or LPS compared with control cultures. Nitrite and nitrate synthesis was substantially blocked by the NOS inhibitor L-NAME, suggesting that iNOS is actively producing NO.
Formal Description
Interaction-ID: 7837

gene/protein

NOS2

increases_quantity of

drug/chemical compound

NO

in primary astrocyte monolayers
Drugbank entries Show/Hide entries for NOS2
Comment Preexposure of astrocytes to peroxynitrite significantly reduced the number of motor neurons surviving on the monolayer. The maximal neurotoxic effect was found after 0.75 mM peroxynitrite, corresponding to a 48% decrease in motor neuron number.
Formal Description
Interaction-ID: 7840

drug/chemical compound

Peroxynitrite

increases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes
Comment The number of motor neurons surviving on the monolayer after exposure to LPS was decreased by 37%.
Formal Description
Interaction-ID: 7841

drug/chemical compound

Lipopolysaccharide

increases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes
Comment INOS inhibitors and superoxide/peroxynitrite scavengers (L-NAME, aminoguanidine, Mn-TBAP, or uric acid) prevented loss of spinal cord motor neurons seeded onto monolayers of astrocytes.
Formal Description
Interaction-ID: 7842

drug/chemical compound

N(G)-Nitro-L-arginine methyl ester

decreases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes; treated with either peroxynitrite or LPS
Comment INOS inhibitors and superoxide/peroxynitrite scavengers (L-NAME, aminoguanidine, Mn-TBAP, or uric acid) prevented loss of spinal cord motor neurons seeded onto monolayers of astrocytes.
Formal Description
Interaction-ID: 7843

drug/chemical compound

Pimagedine hydrochloride

decreases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes; treated with either peroxynitrite or LPS
Comment INOS inhibitors and superoxide/peroxynitrite scavengers (L-NAME, aminoguanidine, Mn-TBAP, or uric acid) prevented loss of spinal cord motor neurons seeded onto monolayers of astrocytes.
Formal Description
Interaction-ID: 7846

drug/chemical compound

MnTBAP

decreases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes; treated with either peroxynitrite or LPS
Comment INOS inhibitors and superoxide/peroxynitrite scavengers (L-NAME, aminoguanidine, Mn-TBAP, or uric acid) prevented loss of spinal cord motor neurons seeded onto monolayers of astrocytes.
Formal Description
Interaction-ID: 7847

drug/chemical compound

Urate

decreases_activity of

phenotype

motor neuron degeneration

of spinal cord motor neurons seeded onto monolayers of astrocytes; treated with either peroxynitrite or LPS